Academic Papers

A Microfluidic Device for Dry Sample Preservation in Remote Settings

Stefano Begolo, Feng Shen and Rustem F. Ismagilov

Lab Chip, 2013,13, 4331-4342

Abstract

This paper describes a microfluidic device for dry preservation of biological specimens at room temperature that incorporates chemical stabilization matrices. Long-term stabilization of samples is crucial for remote medical analysis, biosurveillance, and archiving, but the current paradigm for transporting remotely obtained samples relies on the costly “cold chain” to preserve analytes within biospecimens.

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Live Mammalian Cell Arrays

Kristina Woodruff, Luis M Fidalgo, Samy Gobaa, Matthias P Lutolf, Sebastian J Maerkl,

Nature Methods 10, 550–552 (2013)

Abstract

High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We developed a simple yet powerful method that uses contact spotting to generate high-density nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries.

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Automated Reagent-Dispensing System for Microfluidic Cell Biology Assays

Jimmy Ly, Michael Masterman-Smith, Ravichandran Ramakrishnan, Jing Sun, Brent, Kokubun, R. Michael van Dam.

Journal of Laboratory Automation, 2013, 18,6, 530-541

Abstract

Microscale systems that enable measurements of oncological phenomena at the single-cell level have a great capacity to improve therapeutic strategies and diagnostics. Such measurements can reveal unprecedented insights into cellular heterogeneity and its implications into the progression and treatment of complicated cellular disease processes such as those found in cancer.

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Microfluidic Device for Mechanical Dissociation of Cancer Cell Aggregates into Single Cells.

Xiaolong Qiu, Janice De Jesus, Marissa Pennell, Marco Troiani and Jered B. Haun

Lab Chip, 2015,15, 339-350

Abstract

Tumors tissues house a diverse array of cell types, requiring powerful cell-based analysis methods to characterize different cell subtypes. Tumor tissue is dissociated into single cells by treatment with proteolytic enzymes, followed by mechanical disruption using vortexing or pipetting. These procedures can be incomplete and require significant time, and the latter mechanical treatments are poorly defined and controlled. Here, we present a novel microfluidic device to improve mechanical dissociation of digested tissue and cell aggregates into single cells.

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